What is tryptic soy broth?

Tryptic soy broth (TSB) is a liquid bacterial growth medium used in microbiology and biotechnology laboratories for cultivating a wide variety of microorganisms. It is composed of enzymatic digests of casein and soybean meal, along with additional nutrients like glucose that provide essential amino acids and vitamins for microbial growth.

TSB is a versatile and rich medium that supports the growth of both aerobic and anaerobic bacteria. It is often used for the cultivation and maintenance of bacteria for various applications, including antimicrobial susceptibility testing, bacterial enumeration, and studying bacterial physiology.

TSB is typically sterilized by autoclaving before use to ensure that the medium is free of contaminants. It can be supplemented with additional nutrients or selective agents to facilitate the growth of specific bacterial species or to inhibit the growth of unwanted contaminants.

Overall, TSB is a widely used and reliable medium for the cultivation of a broad range of bacteria and is an essential tool in microbiology research and in clinical and industrial laboratories.